Emodin Down-Regulates Expression of TRPV1 mRNA and Its Function in DRG Neurons in Vitro
Sui, Feng1; Huo, Hai-Ru1; Zhang, Chang-Bin2; Yang, Na1; Guo, Jian-You3; Du, Xin-Liang1; Zhao, Bao-Sheng4; Liu, Hong-Bin1; Li, Lan-Fang1; Guo, Shu-Ying1; Jiang, Ting-Liang1; T.L.Jiang
2010
发表期刊AMERICAN JOURNAL OF CHINESE MEDICINE
ISSN0192-415X
文章类型Article
卷号38期号:4页码:789-800
摘要Emodin is a principle ingredient isolated from rhubarb rhizome, which is commonly used for constipation or pain-related diseases in traditional Chinese medicine (TCM) practice. The transient receptor potential vanilloid 1 ion channel proteins (TRPV1) are abundantly expressed in the peripheral sensory neurons and are assumed to act as a kind of nociceptor involved in the perception of pain and development of hyperalgesia. The aim of this study was to further unravel the analgesic mechanisms of rhubarb through investigating the effects of its main constitutive ingredient emodin on the expression of TRPV1 mRNA as well as on its calcium- mediating functions in vitro. The primary DRG neurons with a high purity and viability were obtained, and the TRPV1 mRNA expression levels were examined by using real-time RT-PCR and the elevated amplitudes of intracellular [Ca2(+)]i in the DRG neurons evoked by TRPV1 agonist capsaicin were examined by confocal microscopy. The results showed that emodin could significantly down-regulate both the mRNA expression of TRPV1 and the capsaicin-evoked intracellular fluorescent intensity in the DRG neurons under both 37 degrees C and 39 degrees C in vitro. Concomitantly, all of the changes induced by emodin could not be blocked by pretreatment of the primary neurons with capsazepine, an antagonist of TRPV1. In conclusion, we established that the mRNA expression level of TRPV1 and its calcium-mediating function in naive DRG neurons could be down-regulated by emodin through perhaps the non-TRPV1 channel pathways, and this might be the molecular mechanisms for rhubarb to inhibit hyperalgesia induced by inflammatory stimuli.
关键词Emodin TRPV1 Analgesic DRG Capsaicin
学科领域行为遗传学
收录类别SCI
语种英语
资助项目一室罗非课题组
WOS记录号WOS:000279796500014
引用统计
文献类型期刊论文
条目标识符http://ir.psych.ac.cn/handle/311026/7021
专题中国科学院心理研究所回溯数据库(1956-2010)
通讯作者T.L.Jiang
作者单位1.China Acad Chinese Med Sci, Inst Chinese Mat Med, Tang Ctr Herbal Med Res, Beijing 100700, Peoples R China
2.Nanjing Univ Chinese Med, Nanjing 210046, Peoples R China
3.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
4.Beijing Univ Chinese Med, Beijing 100102, Peoples R China
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Sui, Feng,Huo, Hai-Ru,Zhang, Chang-Bin,et al. Emodin Down-Regulates Expression of TRPV1 mRNA and Its Function in DRG Neurons in Vitro[J]. AMERICAN JOURNAL OF CHINESE MEDICINE,2010,38(4):789-800.
APA Sui, Feng.,Huo, Hai-Ru.,Zhang, Chang-Bin.,Yang, Na.,Guo, Jian-You.,...&T.L.Jiang.(2010).Emodin Down-Regulates Expression of TRPV1 mRNA and Its Function in DRG Neurons in Vitro.AMERICAN JOURNAL OF CHINESE MEDICINE,38(4),789-800.
MLA Sui, Feng,et al."Emodin Down-Regulates Expression of TRPV1 mRNA and Its Function in DRG Neurons in Vitro".AMERICAN JOURNAL OF CHINESE MEDICINE 38.4(2010):789-800.
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