PSYCH OpenIR  > 中国科学院心理研究所回溯数据库(1956-2010)
Emodin Down-Regulates Expression of TRPV1 mRNA and Its Function in DRG Neurons in Vitro
Sui, Feng1; Huo, Hai-Ru1; Zhang, Chang-Bin2; Yang, Na1; Guo, Jian-You3; Du, Xin-Liang1; Zhao, Bao-Sheng4; Liu, Hong-Bin1; Li, Lan-Fang1; Guo, Shu-Ying1; Jiang, Ting-Liang1; T.L.Jiang
2010
Source PublicationAMERICAN JOURNAL OF CHINESE MEDICINE
ISSN0192-415X
SubtypeArticle
Volume38Issue:4Pages:789-800
AbstractEmodin is a principle ingredient isolated from rhubarb rhizome, which is commonly used for constipation or pain-related diseases in traditional Chinese medicine (TCM) practice. The transient receptor potential vanilloid 1 ion channel proteins (TRPV1) are abundantly expressed in the peripheral sensory neurons and are assumed to act as a kind of nociceptor involved in the perception of pain and development of hyperalgesia. The aim of this study was to further unravel the analgesic mechanisms of rhubarb through investigating the effects of its main constitutive ingredient emodin on the expression of TRPV1 mRNA as well as on its calcium- mediating functions in vitro. The primary DRG neurons with a high purity and viability were obtained, and the TRPV1 mRNA expression levels were examined by using real-time RT-PCR and the elevated amplitudes of intracellular [Ca2(+)]i in the DRG neurons evoked by TRPV1 agonist capsaicin were examined by confocal microscopy. The results showed that emodin could significantly down-regulate both the mRNA expression of TRPV1 and the capsaicin-evoked intracellular fluorescent intensity in the DRG neurons under both 37 degrees C and 39 degrees C in vitro. Concomitantly, all of the changes induced by emodin could not be blocked by pretreatment of the primary neurons with capsazepine, an antagonist of TRPV1. In conclusion, we established that the mRNA expression level of TRPV1 and its calcium-mediating function in naive DRG neurons could be down-regulated by emodin through perhaps the non-TRPV1 channel pathways, and this might be the molecular mechanisms for rhubarb to inhibit hyperalgesia induced by inflammatory stimuli.
KeywordEmodin TRPV1 Analgesic DRG Capsaicin
Subject Area行为遗传学
Indexed BySCI
Language英语
Funding Project一室罗非课题组
WOS IDWOS:000279796500014
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/7021
Collection中国科学院心理研究所回溯数据库(1956-2010)
Corresponding AuthorT.L.Jiang
Affiliation1.China Acad Chinese Med Sci, Inst Chinese Mat Med, Tang Ctr Herbal Med Res, Beijing 100700, Peoples R China
2.Nanjing Univ Chinese Med, Nanjing 210046, Peoples R China
3.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
4.Beijing Univ Chinese Med, Beijing 100102, Peoples R China
Recommended Citation
GB/T 7714
Sui, Feng,Huo, Hai-Ru,Zhang, Chang-Bin,et al. Emodin Down-Regulates Expression of TRPV1 mRNA and Its Function in DRG Neurons in Vitro[J]. AMERICAN JOURNAL OF CHINESE MEDICINE,2010,38(4):789-800.
APA Sui, Feng.,Huo, Hai-Ru.,Zhang, Chang-Bin.,Yang, Na.,Guo, Jian-You.,...&T.L.Jiang.(2010).Emodin Down-Regulates Expression of TRPV1 mRNA and Its Function in DRG Neurons in Vitro.AMERICAN JOURNAL OF CHINESE MEDICINE,38(4),789-800.
MLA Sui, Feng,et al."Emodin Down-Regulates Expression of TRPV1 mRNA and Its Function in DRG Neurons in Vitro".AMERICAN JOURNAL OF CHINESE MEDICINE 38.4(2010):789-800.
Files in This Item:
File Name/Size DocType Version Access License
20101130023747ajcm.p(68KB) 限制开放--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Sui, Feng]'s Articles
[Huo, Hai-Ru]'s Articles
[Zhang, Chang-Bin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Sui, Feng]'s Articles
[Huo, Hai-Ru]'s Articles
[Zhang, Chang-Bin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Sui, Feng]'s Articles
[Huo, Hai-Ru]'s Articles
[Zhang, Chang-Bin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 20101130023747ajcm.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.