Morphine induces Beclin 1-and ATG5-dependent autophagy in human neuroblastoma SH-SY5Y cells and in the rat hippocampus

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	Morphine induces Beclin 1-and ATG5-dependent autophagy in human neuroblastoma SH-SY5Y cells and in the rat hippocampus
	Zhao, Lixia 1,2; Zhu, Yushan 1,3; Wang, Dongmei4 ; Chen, Ming 1; Gao, Ping 1; Xiao, Weiming 5; Rao, Guanhua 3; Wang, Xiaohui 1; Jin, Haijing 1; Xu, Lin 6; Sui, Nan4 ; Chen, Quan 1,3; Sui N(隋南)
摘要	Chronic exposure to morphine can induce drug addiction and neural injury, but the exact mechanism is not fully understood. Here we show that morphine induces autophagy in neuroblastoma SH-SY5Y cells and in the rat hippocampus. Pharmacological approach shows that this effect appears to be mediated by PTX-sensitive G protein-coupled receptors signaling cascade. Morphine increases Beclin 1 expression and reduces the interaction between Beclin 1 and Bcl-2, thus releasing Beclin 1 for its pro-autophagic activity. Bcl-2 overexpression inhibits morphine-induced autophagy, whereas knockdown of Beclin 1 or knockout of ATG5 prevents morphine-induced autophagy. In addition, chronic treatment with morphine induces cell death, which is increased by autophagy inhibition through Beclin 1 RNAi. Our data are the first to reveal that Beclin 1 and ATG5 play key roles in morphine-induced autophagy, which may contribute to morphine-induced neuronal injury.
关键词	morphine autophagy cell death Beclin 1 ATG5
学科领域	医学心理学
	2010-04-01
语种	英语
发表期刊	AUTOPHAGY
ISSN	1554-8627
卷号	6 期号:3 页码:386-394
期刊论文类型	Article
收录类别	SCI
资助项目	一室隋南课题组
WOS记录号	WOS:000276198200009
引用统计	被引频次：62[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.psych.ac.cn/handle/311026/7033
专题	中国科学院心理研究所回溯数据库(1956-2010)
通讯作者	Sui N(隋南)
作者单位	1.Chinese Acad Sci, Inst Zool, State Key Lab Biomembrane & Membrane Biotechnol, Lab Apoptosis & Canc Biol, Beijing 100864, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100864, Peoples R China 3.Nankai Univ, Coll Life Sci, Tianjin, Peoples R China 4.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100864, Peoples R China 5.Peking Univ, Coll Life Sci, Beijing, Peoples R China 6.Chinese Acad Sci, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Kunming, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Lixia,Zhu, Yushan,Wang, Dongmei,et al. Morphine induces Beclin 1-and ATG5-dependent autophagy in human neuroblastoma SH-SY5Y cells and in the rat hippocampus[J]. AUTOPHAGY,2010,6(3):386-394.
APA	Zhao, Lixia.,Zhu, Yushan.,Wang, Dongmei.,Chen, Ming.,Gao, Ping.,...&隋南.(2010).Morphine induces Beclin 1-and ATG5-dependent autophagy in human neuroblastoma SH-SY5Y cells and in the rat hippocampus.AUTOPHAGY,6(3),386-394.
MLA	Zhao, Lixia,et al."Morphine induces Beclin 1-and ATG5-dependent autophagy in human neuroblastoma SH-SY5Y cells and in the rat hippocampus".AUTOPHAGY 6.3(2010):386-394.

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