Human CD1D Gene Expression Is Regulated by LEF-1 through Distal Promoter Regulatory Elements
Chen, Qiao-Yi1; Zhang, Tao2; Pincus, Seth H.2,3; Wu, Shixiu4; Ricks, David2; Liu, Donald6; Sun, Zhongsheng5; Maclaren, Noel7; Lan, Michael S.2,3
2010-05-01
发表期刊JOURNAL OF IMMUNOLOGY
ISSN0022-1767
文章类型Article
卷号184期号:9页码:5047-5054
摘要CD1d-expressing cells present lipid Ag to CD1d-restricted NKT cells, which play an important role in immune regulation and tumor rejection. Lymphoid enhancer-binding factor-1 (LEF-1) is one of the regulators of the Wnt signaling pathway, which is a powerful regulator in cellular growth, differentiation, and transformation. There is little evidence connecting Wnt signaling to CD1d expression. In this study, we have identified LEF-1 as a regulator of the expression of the gene encoding the human CD1d molecule (CD1D). We found that LEF-1 binds specifically to the CD1D promoter. Overexpression of LEF-1 in K562 or Jurkat cells suppresses CD1D promoter activity and downregulates endogenous CD1D transcripts, whereas knockdown of LEF-1 using LEF-1 specific small interfering RNA increases CD1D transcripts in K562 and Jurkat cells but there are different levels of surface CD1d on these two cell types. Chromatin immunoprecipitation showed that the endogenous LEF-1 is situated at the CD1D promoter and interacts with histone deacetylase-1 to facilitate the transcriptional repressor activity. Knockdown of LEF-1 using small interfering RNA potentiates an acetylation state of histone H3/H4, supporting the notion that LEF-1 acts as a transcriptional repressor for the CD1D gene. Our finding links LEF-1 to CD1D and suggests a role of Wnt signaling in the regulation of the human CD1D gene. The Journal of Immunology, 2010, 184: 5047-5054.
学科领域行为遗传学
收录类别SCI
语种英语
资助项目一室原孙中生课题组
WOS记录号WOS:000277093000057
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.psych.ac.cn/handle/311026/7037
专题中国科学院心理研究所回溯数据库(1956-2010)
作者单位1.Qugene Corp, Metairie, LA 70003 USA
2.Childrens Hosp, Res Inst Children, New Orleans, LA 70118 USA
3.Louisiana State Univ, Hlth Sci Ctr, Dept Pediat, New Orleans, LA 70112 USA
4.Wenzhou Med Coll, Affiliated Hosp 1, Dept Radiotherapy, Wenzhou, Peoples R China
5.Chinese Acad Sci, Inst Psychol, Beijing, Peoples R China
6.Univ Chicago, Dept Surg, Chicago, IL 60637 USA
7.BioSeek Endocrinol Clin, New York, NY 10022 USA
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Chen, Qiao-Yi,Zhang, Tao,Pincus, Seth H.,et al. Human CD1D Gene Expression Is Regulated by LEF-1 through Distal Promoter Regulatory Elements[J]. JOURNAL OF IMMUNOLOGY,2010,184(9):5047-5054.
APA Chen, Qiao-Yi.,Zhang, Tao.,Pincus, Seth H..,Wu, Shixiu.,Ricks, David.,...&Lan, Michael S..(2010).Human CD1D Gene Expression Is Regulated by LEF-1 through Distal Promoter Regulatory Elements.JOURNAL OF IMMUNOLOGY,184(9),5047-5054.
MLA Chen, Qiao-Yi,et al."Human CD1D Gene Expression Is Regulated by LEF-1 through Distal Promoter Regulatory Elements".JOURNAL OF IMMUNOLOGY 184.9(2010):5047-5054.
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