Human CD1D Gene Expression Is Regulated by LEF-1 through Distal Promoter Regulatory Elements

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	Human CD1D Gene Expression Is Regulated by LEF-1 through Distal Promoter Regulatory Elements
	Chen, Qiao-Yi 1; Zhang, Tao2 ; Pincus, Seth H.2,3; Wu, Shixiu 4; Ricks, David 2; Liu, Donald 6; Sun, Zhongsheng5 ; Maclaren, Noel 7; Lan, Michael S.2,3
摘要	CD1d-expressing cells present lipid Ag to CD1d-restricted NKT cells, which play an important role in immune regulation and tumor rejection. Lymphoid enhancer-binding factor-1 (LEF-1) is one of the regulators of the Wnt signaling pathway, which is a powerful regulator in cellular growth, differentiation, and transformation. There is little evidence connecting Wnt signaling to CD1d expression. In this study, we have identified LEF-1 as a regulator of the expression of the gene encoding the human CD1d molecule (CD1D). We found that LEF-1 binds specifically to the CD1D promoter. Overexpression of LEF-1 in K562 or Jurkat cells suppresses CD1D promoter activity and downregulates endogenous CD1D transcripts, whereas knockdown of LEF-1 using LEF-1 specific small interfering RNA increases CD1D transcripts in K562 and Jurkat cells but there are different levels of surface CD1d on these two cell types. Chromatin immunoprecipitation showed that the endogenous LEF-1 is situated at the CD1D promoter and interacts with histone deacetylase-1 to facilitate the transcriptional repressor activity. Knockdown of LEF-1 using small interfering RNA potentiates an acetylation state of histone H3/H4, supporting the notion that LEF-1 acts as a transcriptional repressor for the CD1D gene. Our finding links LEF-1 to CD1D and suggests a role of Wnt signaling in the regulation of the human CD1D gene. The Journal of Immunology, 2010, 184: 5047-5054.
学科领域	行为遗传学
	2010-05-01
语种	英语
发表期刊	JOURNAL OF IMMUNOLOGY
ISSN	0022-1767
卷号	184 期号:9 页码:5047-5054
期刊论文类型	Article
收录类别	SCI
资助项目	一室原孙中生课题组
WOS记录号	WOS:000277093000057
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.psych.ac.cn/handle/311026/7037
专题	中国科学院心理研究所回溯数据库(1956-2010)
作者单位	1.Qugene Corp, Metairie, LA 70003 USA 2.Childrens Hosp, Res Inst Children, New Orleans, LA 70118 USA 3.Louisiana State Univ, Hlth Sci Ctr, Dept Pediat, New Orleans, LA 70112 USA 4.Wenzhou Med Coll, Affiliated Hosp 1, Dept Radiotherapy, Wenzhou, Peoples R China 5.Chinese Acad Sci, Inst Psychol, Beijing, Peoples R China 6.Univ Chicago, Dept Surg, Chicago, IL 60637 USA 7.BioSeek Endocrinol Clin, New York, NY 10022 USA
推荐引用方式 GB/T 7714	Chen, Qiao-Yi,Zhang, Tao,Pincus, Seth H.,et al. Human CD1D Gene Expression Is Regulated by LEF-1 through Distal Promoter Regulatory Elements[J]. JOURNAL OF IMMUNOLOGY,2010,184(9):5047-5054.
APA	Chen, Qiao-Yi.,Zhang, Tao.,Pincus, Seth H..,Wu, Shixiu.,Ricks, David.,...&Lan, Michael S..(2010).Human CD1D Gene Expression Is Regulated by LEF-1 through Distal Promoter Regulatory Elements.JOURNAL OF IMMUNOLOGY,184(9),5047-5054.
MLA	Chen, Qiao-Yi,et al."Human CD1D Gene Expression Is Regulated by LEF-1 through Distal Promoter Regulatory Elements".JOURNAL OF IMMUNOLOGY 184.9(2010):5047-5054.

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