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The Trw Type IV Secretion System of Bartonella Mediates Host-Specific Adhesion to Erythrocytes
Vayssier-Taussat, Muriel1,2; Le Rhun, Danielle1; Deng, Hong Kuan1; Biville, Francis3; Cescau, Sandra4; Danchin, Antoine5; Marignac, Genevieve1; Lenaour, Evelyne1; Boulouis, Henri Jean3; Mavris, Maria1; Arnaud, Lionel6; Yang, Huanming7; Wang, Jing8; Quebatte, Maxime2; Engel, Philipp2; Saenz, Henri2; Dehio, Christoph2; Christoph Dehio
2010-06-01
Source PublicationPLOS PATHOGENS
ISSN1553-7366
SubtypeArticle
Volume6Issue:6
AbstractBacterial pathogens typically infect only a limited range of hosts; however, the genetic mechanisms governing host-specificity are poorly understood. The alpha-proteobacterial genus Bartonella comprises 21 species that cause host-specific intraerythrocytic bacteremia as hallmark of infection in their respective mammalian reservoirs, including the human-specific pathogens Bartonella quintana and Bartonella bacilliformis that cause trench fever and Oroya fever, respectively. Here, we have identified bacterial factors that mediate host-specific erythrocyte colonization in the mammalian reservoirs. Using mouse-specific Bartonella birtlesii, human-specific Bartonella quintana, cat-specific Bartonella henselae and rat-specific Bartonella tribocorum, we established in vitro adhesion and invasion assays with isolated erythrocytes that fully reproduce the host-specificity of erythrocyte infection as observed in vivo. By signature-tagged mutagenesis of B. birtlesii and mutant selection in a mouse infection model we identified mutants impaired in establishing intraerythrocytic bacteremia. Among 45 abacteremic mutants, five failed to adhere to and invade mouse erythrocytes in vitro. The corresponding genes encode components of the type IV secretion system (T4SS) Trw, demonstrating that this virulence factor laterally acquired by the Bartonella lineage is directly involved in adherence to erythrocytes. Strikingly, ectopic expression of Trw of rat-specific B. tribocorum in cat-specific B. henselae or human-specific B. quintana expanded their host range for erythrocyte infection to rat, demonstrating that Trw mediates host-specific erythrocyte infection. A molecular evolutionary analysis of the trw locus further indicated that the variable, surface-located TrwL and TrwJ might represent the T4SS components that determine host-specificity of erythrocyte parasitism. In conclusion, we show that the laterally acquired Trw T4SS diversified in the Bartonella lineage to facilitate host-restricted adhesion to erythrocytes in a wide range of mammals.
Subject Area生理心理学/生物心理学
Indexed BySCI
Language英语
Funding Project一室王晶课题组
WOS IDWOS:000279806300020
Citation statistics
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/7269
Collection中国科学院心理研究所回溯数据库(1956-2010)
Corresponding AuthorChristoph Dehio
Affiliation1.INRA, Unite Contrat Bartonella, Maisons Alfort, France
2.Univ Basel, Biozentrum, Focal Area Infect Biol, Basel, Switzerland
3.ENVA AFSSA, Equipe Bacteries Zoonot Hemotropes, UMR BIPAR, Maisons Alfort, France
4.Inst Pasteur, CNRS, URA 2171, Unite Genomes Bacteriens, Paris, France
5.AMAbiotics, Evry, France
6.Inst Natl Transfus Sanguine, F-75015 Paris, France
7.Beijing Genom Inst, Shenzhen, Peoples R China
8.Chinese Acad Sci, Inst Psychol, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Vayssier-Taussat, Muriel,Le Rhun, Danielle,Deng, Hong Kuan,et al. The Trw Type IV Secretion System of Bartonella Mediates Host-Specific Adhesion to Erythrocytes[J]. PLOS PATHOGENS,2010,6(6).
APA Vayssier-Taussat, Muriel.,Le Rhun, Danielle.,Deng, Hong Kuan.,Biville, Francis.,Cescau, Sandra.,...&Christoph Dehio.(2010).The Trw Type IV Secretion System of Bartonella Mediates Host-Specific Adhesion to Erythrocytes.PLOS PATHOGENS,6(6).
MLA Vayssier-Taussat, Muriel,et al."The Trw Type IV Secretion System of Bartonella Mediates Host-Specific Adhesion to Erythrocytes".PLOS PATHOGENS 6.6(2010).
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