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How to make a minimal genome for synthetic minimal cell
Zhang, Liu-yan; Chang, Su-hua; Wang, Jing; Wang J(王晶)
Source PublicationPROTEIN & CELL
AbstractAs a key focus of synthetic biology, building a minimal artificial cell has given rise to many discussions. A synthetic minimal cell will provide an appropriate chassis to integrate functional synthetic parts, devices and systems with functions that cannot generally be found in nature. The design and construction of a functional minimal genome is a key step while building such a cell/chassis since all the cell functions can be traced back to the genome. Kinds of approaches, based on bioinformatics and molecular biology, have been developed and proceeded to derive essential genes and minimal gene sets for the synthetic minimal genome. Experiments about streamlining genomes of model bacteria revealed genome reduction led to unanticipated beneficial properties, such as high electroporation efficiency and accurate propagation of recombinant genes and plasmids that were unstable in other strains. Recent achievements in chemical synthesis technology for large DNA segments together with the rapid development of the whole-genome sequencing, have transferred synthesis of genes to assembly of the whole genomes based on oligonucleotides, and thus created strong preconditions for synthesis of artificial minimal genome. Here in this article, we review briefly the history and current state of research in this field and summarize the main methods for making a minimal genome. We also discuss the impacts of minimized genome on metabolism and regulation of artificial cell.
Keywordsynthetic biology minimal genome essential gene minimal cell
Subject Area生理心理学/生物心理学
Indexed BySCI
Funding Project一室王晶课题组
WOS IDWOS:000208510900005
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorWang J(王晶)
AffiliationChinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
First Author AffilicationKey Laboratory of Mental Health, CAS
Recommended Citation
GB/T 7714
Zhang, Liu-yan,Chang, Su-hua,Wang, Jing,et al. How to make a minimal genome for synthetic minimal cell[J]. PROTEIN & CELL,2010,1(5):427-434.
APA Zhang, Liu-yan,Chang, Su-hua,Wang, Jing,&王晶.(2010).How to make a minimal genome for synthetic minimal cell.PROTEIN & CELL,1(5),427-434.
MLA Zhang, Liu-yan,et al."How to make a minimal genome for synthetic minimal cell".PROTEIN & CELL 1.5(2010):427-434.
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