How to make a minimal genome for synthetic minimal cell

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	How to make a minimal genome for synthetic minimal cell
	Zhang, Liu-yan; Chang, Su-hua; Wang, Jing; Wang J(王晶)
摘要	As a key focus of synthetic biology, building a minimal artificial cell has given rise to many discussions. A synthetic minimal cell will provide an appropriate chassis to integrate functional synthetic parts, devices and systems with functions that cannot generally be found in nature. The design and construction of a functional minimal genome is a key step while building such a cell/chassis since all the cell functions can be traced back to the genome. Kinds of approaches, based on bioinformatics and molecular biology, have been developed and proceeded to derive essential genes and minimal gene sets for the synthetic minimal genome. Experiments about streamlining genomes of model bacteria revealed genome reduction led to unanticipated beneficial properties, such as high electroporation efficiency and accurate propagation of recombinant genes and plasmids that were unstable in other strains. Recent achievements in chemical synthesis technology for large DNA segments together with the rapid development of the whole-genome sequencing, have transferred synthesis of genes to assembly of the whole genomes based on oligonucleotides, and thus created strong preconditions for synthesis of artificial minimal genome. Here in this article, we review briefly the history and current state of research in this field and summarize the main methods for making a minimal genome. We also discuss the impacts of minimized genome on metabolism and regulation of artificial cell.
关键词	synthetic biology minimal genome essential gene minimal cell
学科领域	生理心理学/生物心理学
	2010-05-01
语种	英语
发表期刊	PROTEIN & CELL
ISSN	1674-800X
卷号	1 期号:5 页码:427-434
期刊论文类型	Review
收录类别	SCI
资助项目	一室王晶课题组
WOS记录号	WOS:000208510900005
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.psych.ac.cn/handle/311026/7275
专题	中国科学院心理研究所回溯数据库(1956-2010)
通讯作者	Wang J(王晶)
作者单位	Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
第一作者单位	中国科学院心理健康重点实验室
推荐引用方式 GB/T 7714	Zhang, Liu-yan,Chang, Su-hua,Wang, Jing,et al. How to make a minimal genome for synthetic minimal cell[J]. PROTEIN & CELL,2010,1(5):427-434.
APA	Zhang, Liu-yan,Chang, Su-hua,Wang, Jing,&王晶.(2010).How to make a minimal genome for synthetic minimal cell.PROTEIN & CELL,1(5),427-434.
MLA	Zhang, Liu-yan,et al."How to make a minimal genome for synthetic minimal cell".PROTEIN & CELL 1.5(2010):427-434.

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