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Optimization of Zygosity Determination by Questionnaire and DNA Genotyping in Chinese Adolescent Twins
Chen, Jie1; Li, Xinying1; Chen, Zhiyan1; Yang, Xiaodong1; Zhang, Jie1; Duan, Qing1; Ge, Xiaojia1,2; Li XY(李新影)
2010-04-01
Source PublicationTWIN RESEARCH AND HUMAN GENETICS
ISSN1832-4274
SubtypeArticle
Volume13Issue:2Pages:194-200
AbstractThe main aim of this study was to develop and optimize a questionnaire-based zygosity determination method in Chinese adolescent twins. Participants were 471 pairs of same-sex twins (345 monozygotic, 126 dizygotic) with a mean age of 14.56 years (SD = 2.62). A second sample was recruited for cross-validation, including 382 pairs of same-sex twins (261 monozygotic, 121 dizygotic) with a mean age of 12.53 years (SD = 2.22). The questionnaire consisted of 12 questions dealing with co-twin similarity or frequency of confusion. Two means were put forward to improve the predictive accuracy of the questionnaire adding parent-reports to the analysis, and using a 2-point rather than 3-point response format. DNA genotyping was performed on nine short tandem repeat loci, with an estimated zygosity classification accuracy very close to 100%. The validity of all questionnaires was assessed by being compared to the results of DNA analysis. Results of stepwise logistic regression analysis showed that the predictive accuracy of the 3-point self-reported questionnaire is 83.8%. Using parent-reports and 2-point scale led to 3.9% and 4.6% increase in predictive accuracy, respectively. When using the parent-reports and children's self-reports jointly, the predictive accuracy was enhanced to 90.6%. For the cross-validation, the equations and cut-offs derived from the first sample led to an acceptable accuracy (91.3%) in the second sample. In conclusion, the method we developed can be used in future studies among Chinese adolescent twins. Multiple-rater and 2-point response format were suggested for all twin studies for enhancing the predictive accuracy of questionnaires.
Keywordzygosity determination in Chinese adolescent twins
Subject Area心理测量学
Indexed BySCI ; SSCI
Language英语
Funding Project一室李新颖课题组
WOS IDWOS:000287994500008
Citation statistics
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/7317
Collection中国科学院心理研究所回溯数据库(1956-2010)
Corresponding AuthorLi XY(李新影)
Affiliation1.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
2.Univ Minnesota Twin Cities, Inst Child Dev, Minneapolis, MN 55455 USA
First Author AffilicationKey Laboratory of Mental Health, CAS
Recommended Citation
GB/T 7714
Chen, Jie,Li, Xinying,Chen, Zhiyan,et al. Optimization of Zygosity Determination by Questionnaire and DNA Genotyping in Chinese Adolescent Twins[J]. TWIN RESEARCH AND HUMAN GENETICS,2010,13(2):194-200.
APA Chen, Jie.,Li, Xinying.,Chen, Zhiyan.,Yang, Xiaodong.,Zhang, Jie.,...&李新影.(2010).Optimization of Zygosity Determination by Questionnaire and DNA Genotyping in Chinese Adolescent Twins.TWIN RESEARCH AND HUMAN GENETICS,13(2),194-200.
MLA Chen, Jie,et al."Optimization of Zygosity Determination by Questionnaire and DNA Genotyping in Chinese Adolescent Twins".TWIN RESEARCH AND HUMAN GENETICS 13.2(2010):194-200.
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