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抗炎性细胞因子IL-10 与IL-4 对大鼠抑郁行为的作用
Alternative TitleEffects of anti-inflammatory cytokines IL-10 and IL-4 on depressive behavior in rats
Thesis Advisor林文娟
Degree Grantor中国科学院研究生院
Place of Conferral北京
Degree Discipline心理学
Keyword抗炎性细胞因子 白介素-10 白介素-4 抑郁症 抑郁行为
研究目的:本研究的目的是观察抗炎性细胞因子白介素-10(Interleukin-10,IL-10)与白介素-4(Interleukin-4,IL-4)能否逆转持续数小时至24 小时的动物抑郁行为,并观察IL-10 的抗抑郁作用是否受到干预时间的影响。
研究方法:本研究采用中枢三次注射细菌脂多糖(Lipopolysaccharide,LPS)的大鼠抑郁模型,该模型的抑郁行为可从数小时持续到24 小时。首先以IL-10 和IL-4 为干预药物,在每次注射LPS 前2 小时干预,观察其对大鼠的体重变化、糖精水偏好、旷场测试运动距离和直立次数的影响。然后比较在注射LPS 前12小时与前2 小时使用IL-10 干预,对大鼠的体重变化、糖精水偏好、旷场测试活动距离和直立次数、及悬尾测试不动时间的影响。
结果:IL-10 能够改善部分大鼠中枢三次注射LPS 所致的快感缺乏和活动水平降
低的症状。IL-4 对大鼠中枢三次注射LPS 所致的快感缺乏无影响,但能够改善部分大鼠活动水平降低的症状。在注射LPS 前12 小时用IL-10 干预,比起提前2 小时用IL-10 干预,对部分大鼠抑郁行为的改善效果更优。
结论:本研究验证了抗炎性细胞因子IL-10 的抗抑郁作用,但并不是对全部动物都有效。IL-10 的抗抑郁效果与给药时间有关。
Other AbstractBackground: The cytokine hypothesis of depression has received considerable research attention for the mechanism study and the therapeutic treatments of depressive disorders. While pro-inflammatory cytokines have been demonstrated to play an important role in the pathological mechanism of depression , the anti- inflammatory cytokines are suggested to have some effects on alleviating depression-related symptoms. However, the animal models used for examination of the effects of anti- inflammatory cytokines on behavioral depression often last only a few hours, which can hardly be regarded as a model of depression. Moreover, whether the anti-depressant effects of anti- inflammatory cytokine are affected by the time of
administration is still unclear.
Objective: The purpose of this study was to investigate whether anti- inflammatory cytokines interleukin-10 (IL-10) and interleukin-4 (IL-4) can reverse the depressive behaviors which up to 24 hours, and whether the reversal effects of anti- inflammatory cytokines were affected by the administration time.
Methods: In this study, a rat model of depression induced by repeated central lipopolysaccharide(LPS) administration was used, which can induce significant depressive-like behaviors after 24 hours of the last LPS injection. Firstly, IL-10 or IL-4 was injected ICV 2 hours before LPS administration and its effects on the LPS-induced changes in body weight, saccharin preference, and open- field test was observed. Then IL-10 was injected ICV 12 hours of 2 hours before LPS was administered, and the changes in body, saccharin preference, open field test, and tail suspension test was measured.
Results: LPS-induced anhedonia and decreased motor activities can be reversed by IL-10 in some rats. IL-4 has no effect on anhedonia induced by LPS, yet it can improve motor activities in some rats. And ICV injection of IL-10 12 hours before LPS showed better anti-depressive effects than 2 hours.
Conclusions: This study indicated anti- inflammatory cytokine IL-10 has some antidepressant effect, though not in all the rats. It also suggests that IL-10's pharmacological efficacy may depend on its intervention time.
Subject Area医学心理学
Document Type学位论文
Recommended Citation
GB/T 7714
徐说. 抗炎性细胞因子IL-10 与IL-4 对大鼠抑郁行为的作用[D]. 北京. 中国科学院研究生院,2014.
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