The role of retinal dopamine in C57BL/6 mouse refractive development as revealed by intravitreal administration of 6-hydroxydopamine
Xiao-Hua Wu; Kang-Wei Qian; Guo-Zhong Xu; Yun-Yun Li; Yuan-Yuan Ma; Furong Huang; Yan-Qing Wang; Xiangtian Zhou; Jia Qu; Xiong-Li Yang; Yong-Mei Zhong; Shi-Jun Weng
Conference Name2016年第一届北京视觉科学会议
Conference Date2016-07
Conference Place北京

PURPOSE: While retinal dopamine (DA) has been long implicated in myopia development, current studies demonstrate that retinal DA levels are unaltered in C57BL/6 mice with form-deprivation myopia. This work was undertaken to explore whether and how refractive development is perturbed in this mouse strain when retinal DA levels are reduced by 6-hydroxydopamine (6-OHDA) administration.
METHODS: 6-OHDA of different doses was intravitreally applied. Retinal DA levels were measured by HPLC and TH levels analyzed by quantitative Western blotting. To choose appropriate 6-OHDA doses that significantly reduce retinal DA levels, but caused minimal disturbance of overall retinal physiology, ERG analysis was performed. Refractive errors were measured using a photorefractor, and ocular biometry assessed with optical coherence tomography and photokeratometry.
RESULTS: 6-OHDA of 6.25 μg and 12.5 μg significantly reduced retinal levels of DA and TH, but did not significantly affect ERG a- and b-wave amplitudes. With normal visual experience, 6-OHDA induced myopic refractive shifts in a dose-dependent fashion. Form-deprivation induced further myopic shifts in 6-OHDA injected eyes, but did not cause further decline in retinal DA. Furthermore, 6-OHDA administration resulted in a shorter axial length and a steeper cornea, while form-deprivation led to a longer axial length, without changing the corneal radius of curvature.
CONCLUSIONS: Reducing retinal DA levels by 6-OHDA led to myopic refractive shifts in C57BL/6 mice, which mainly resulted from a steeper cornea. We propose that, in addition to the DA-independent mechanism for form-deprivation induced myopic refractive shifts, there is a DA-dependent mechanism in parallel that underlies myopic refractive shifts in this mouse strain.

Funding Organization中国科学院心理研究所
Keywordrefractive development form-deprivation myopia dopamine C57BL/6 mouse 6-hydroxydopamine
Subject Area感知觉心理学
Indexed By其他
Document Type会议论文
Affiliation1.Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, China, 200032
2.Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China, 200032
3.School of Ophthalmology and Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China, 325027
Recommended Citation
GB/T 7714
Xiao-Hua Wu,Kang-Wei Qian,Guo-Zhong Xu,et al. The role of retinal dopamine in C57BL/6 mouse refractive development as revealed by intravitreal administration of 6-hydroxydopamine[C]. 见:2016年第一届北京视觉科学会议. 北京. 2016-07.
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