Retinal Ganglion Cell Responses to Low-frequency Focused Ultrasound Stimulation
Huixia Zhao; Qiuju Jiang; Guofeng Li; Weibao Qiu
Conference Name2017年第二届曲阜视觉科学会议
Conference Date2017.7.1
Conference Place曲阜

PURPOSE: Acoustic retinal prosthesis has been put forward using high-frequency US with non-invasive and high-resolution advantages. But its application is limited by the difficulties in fabrication, energy consumption and acoustic attenuation. In the present study, low-frequency focused ultrasound stimulation (LFUS) had been demonstrated to activate retinal ganglion cells (RGCs) in rat retinas. The neurophysiological properties of RGC responses to LFUS were also investigated.
METHODS: A 2.25 MHz focused ultrasound transducer (D=0.75 in., SF=2.0 in.) was used to stimulate the rat retina which was cultured in a multi-electrode array system (MEA2100, MCS , Fig. 1). The acoustic property was evaluated by hydrophone (UMS3, Precision acoustics). Ultrasound (US) stimulation was modulated at pulsed mode. Light stimulation was modulated in the same mode to give an uniform field flashes. The electrophysiological data collected from MEA was detected for neural spikes and sorted by Plexon Offline Sorter. Only channels recording single-cell activities were adopted for subsequent analysis. Peri-stimulus time histograms and raster plots were plotted for each RGC using Spike 2.
RESULTS: In total, 116 retinal ganglion cells (RGCs) from 7 retinas were sorted and classified into four types according to light responses. The firing activity of 114 RGCs were modulated by repeated US stimulation. This suggested that low-frequency focused ultrasound stimulation (LFUS) could activate RGCs. The US responses didn’t correspond to the standard light responses and varied greatly between cell types. Besides, dual-peak responses to US stimulation were observed which were not reported previously. The temporal response properties of RGCs, including latency, firing rate, and response type, could be modulated by changing acoustic intensity.
CONCLUSIONS: These findings might imply a temporal neuromodulation effect of LFUS and provided an important foundation for the development of acoustic retinal prosthesis.

Keywordretinal ganglion cell ultrasound stimulation acoustic retinal prosthesis
Subject Area视觉神经科学
Document Type会议论文
AffiliationLauterbur Research Center for Biomedical Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Huixia Zhao,Qiuju Jiang,Guofeng Li,et al. Retinal Ganglion Cell Responses to Low-frequency Focused Ultrasound Stimulation[C],2017.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Huixia Zhao]'s Articles
[Qiuju Jiang]'s Articles
[Guofeng Li]'s Articles
Baidu academic
Similar articles in Baidu academic
[Huixia Zhao]'s Articles
[Qiuju Jiang]'s Articles
[Guofeng Li]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Huixia Zhao]'s Articles
[Qiuju Jiang]'s Articles
[Guofeng Li]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.