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Alantolactone exhibits selective antitumor effects in HELA human cervical cancer cells by inhibiting cell migration and invasion, G2/M cell cycle arrest, mitochondrial mediated apoptosis and targeting Nf-kB signalling pathway
Yibing Zhang1; Zhao, Yong2; Ran, Yongwang3; Guo, Jianyou4; Cui, Haifeng2; Liu, Sha1
第一作者Yibing Zhang
通讯作者邮箱stevelmartinrr222@yahoo.com
心理所单位排序4
摘要

Purpose: Cervical cancer is responsible for significant mortality and morbidity across the globe. Owing to the adverse effects of the currently used chemotherapy, research is directed to develop effective and safer chemotherapy for cervical cancer. This study was therefore designed to examine the effects of Alantolactone (AL) against a panel of human cervical cancer cells (HeLa, C4-1, MEK-180, C33A) and a normal cell line (HCerEPiC). Methods: Cell viability was examined by WST-1 assay. Cell migration and invasion were examined by transwell assay. Cell cycle analysis was performed by flow cytometry. Apoptosis was detected by annexin V/propidium iodide (PI) and DAPI staining. Western blot analysis was used to examine protein expression. Results: The results revealed that AL inhibits the growth of the all the cervical cancer cells in a concentration -dependent manner and exhibited the lowest IC50 of 15 mu M against the HeLa cervical cancer cells. The anticancer effects of AL were due to induction of mitochondrial mediated apoptosis. AL caused enhancement in the expression of apoptosis regulatory proteins such as caspase 3 and Bax in cervical cancer cells. AL not only prompted the accumulation of the cervical cancer cells in the G2/M phase of the cell cycle but also inhibited the expressions of various cyclins. Transwell assay revealed that AL suppresses the migration and invasion of cervical cancer cells. Moreover AL could also block the NF-kB signalling pathway concentration-dependently. Conclusions: It is concluded that AL may serve as an important lead molecule for the development of therapy for cervical cancer.

关键词alantolactone apoptosis cervical cancer cytometry cell cycle
2019-11-01
语种英语
发表期刊JOURNAL OF BUON
ISSN1107-0625
卷号24期号:6页码:2310-2315
期刊论文类型article
收录类别SCI
出版者IMPRIMATUR PUBLICATIONS
WOS关键词NATURAL-PRODUCTS ; WOMEN
WOS研究方向Oncology
WOS类目Oncology
WOS记录号WOS:000503405400015
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.psych.ac.cn/handle/311026/30558
专题健康与遗传心理学研究室
通讯作者Liu, Sha
作者单位1.Chongqing Med Univ, Sch Tradit Chinese Med, Chongqing 401331, Peoples R China
2.China Acad Tradit Chinese Med, Inst Chinese Mat Med, Beijing 100700, Peoples R China
3.Qianjiang Cent Hosp Chongqing, Chongqing 409099, Peoples R China
4.Chinese Acad Sci, Inst Psychol, Beijing 100101, Peoples R China
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Yibing Zhang,Zhao, Yong,Ran, Yongwang,et al. Alantolactone exhibits selective antitumor effects in HELA human cervical cancer cells by inhibiting cell migration and invasion, G2/M cell cycle arrest, mitochondrial mediated apoptosis and targeting Nf-kB signalling pathway[J]. JOURNAL OF BUON,2019,24(6):2310-2315.
APA Yibing Zhang,Zhao, Yong,Ran, Yongwang,Guo, Jianyou,Cui, Haifeng,&Liu, Sha.(2019).Alantolactone exhibits selective antitumor effects in HELA human cervical cancer cells by inhibiting cell migration and invasion, G2/M cell cycle arrest, mitochondrial mediated apoptosis and targeting Nf-kB signalling pathway.JOURNAL OF BUON,24(6),2310-2315.
MLA Yibing Zhang,et al."Alantolactone exhibits selective antitumor effects in HELA human cervical cancer cells by inhibiting cell migration and invasion, G2/M cell cycle arrest, mitochondrial mediated apoptosis and targeting Nf-kB signalling pathway".JOURNAL OF BUON 24.6(2019):2310-2315.
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