PSYCH OpenIR  > 中国科学院心理研究所回溯数据库(1956-2010)
mirTools: microRNA profiling and discovery based on high-throughput sequencing
Zhu, Erle1,2; Zhao, Fangqing3; Xu, Gang2; Hou, Huabin2; Zhou, LingLin2; Li, Xiaokun1; Sun, Zhongsheng2,4; Wu, Jinyu2; Sun ZS(孙中生); Xiaokun Li; Jinyu Wu
2010-07-01
Source PublicationNUCLEIC ACIDS RESEARCH
ISSN0305-1048
SubtypeArticle
Volume38Issue:1Pages:W392-W397
AbstractmiRNAs are small, non-coding RNA that negatively regulate gene expression at post-transcriptional level, which play crucial roles in various physiological and pathological processes, such as development and tumorigenesis. Although deep sequencing technologies have been applied to investigate various small RNA transcriptomes, their computational methods are far away from maturation as compared to microarray-based approaches. In this study, a comprehensive web server mirTools was developed to allow researchers to comprehensively characterize small RNA transcriptome. With the aid of mirTools, users can: (i) filter low-quality reads and 3/5' adapters from raw sequenced data; (ii) align large-scale short reads to the reference genome and explore their length distribution; (iii) classify small RNA candidates into known categories, such as known miRNAs, non-coding RNA, genomic repeats and coding sequences; (iv) provide detailed annotation information for known miRNAs, such as miRNA/miRNA*, absolute/relative reads count and the most abundant tag; (v) predict novel miRNAs that have not been characterized before; and (vi) identify differentially expressed miRNAs between samples based on two different counting strategies: total read tag counts and the most abundant tag counts. We believe that the integration of multiple computational approaches in mirTools will greatly facilitate current microRNA researches in multiple ways. mirTools can be accessed at http://centre.bioinformatics.zj.cn/mirtools/ and http://59.79.168.90/mirtools.
Subject Area生理心理学/生物心理学
Indexed BySCI
Language英语
Funding Project一室原孙中生课题组
WOS IDWOS:000284148900062
Citation statistics
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/7249
Collection中国科学院心理研究所回溯数据库(1956-2010)
Corresponding AuthorSun ZS(孙中生); Xiaokun Li; Jinyu Wu
Affiliation1.Wenzhou Med Coll, Zhejiang Prov Key Lab Biotechnol Pharmaceut Engn, Sch Pharmaceut Sci, Wenzhou 325035, Peoples R China
2.Wenzhou Med Coll, Zhejiang Prov Key Lab Med Genet, Inst Genom Med, Wenzhou 325035, Peoples R China
3.Penn State Univ, Ctr Comparat Genom & Bioinformat, Dept Biochem & Mol Biol, University Pk, PA 16802 USA
4.Chinese Acad Sci, Inst Psychol, Behav Genet Ctr, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Erle,Zhao, Fangqing,Xu, Gang,et al. mirTools: microRNA profiling and discovery based on high-throughput sequencing[J]. NUCLEIC ACIDS RESEARCH,2010,38(1):W392-W397.
APA Zhu, Erle.,Zhao, Fangqing.,Xu, Gang.,Hou, Huabin.,Zhou, LingLin.,...&Jinyu Wu.(2010).mirTools: microRNA profiling and discovery based on high-throughput sequencing.NUCLEIC ACIDS RESEARCH,38(1),W392-W397.
MLA Zhu, Erle,et al."mirTools: microRNA profiling and discovery based on high-throughput sequencing".NUCLEIC ACIDS RESEARCH 38.1(2010):W392-W397.
Files in This Item:
File Name/Size DocType Version Access License
20101202040518mirtoo(5117KB) 限制开放--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhu, Erle]'s Articles
[Zhao, Fangqing]'s Articles
[Xu, Gang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhu, Erle]'s Articles
[Zhao, Fangqing]'s Articles
[Xu, Gang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhu, Erle]'s Articles
[Zhao, Fangqing]'s Articles
[Xu, Gang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 20101202040518mirtools microrna profiling and discovery based on high throughput sequencing.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.