中国科学院心理健康重点实验室知识库

目的: 对4 种刚柔证造模方法进行客观评价，初步筛选出刚柔证造模方法。方法: 将40 只SD 大鼠随机分为5 组，分别予以睡眠剥夺造模法、社交失败造模法、束缚造模法、长期慢性不确定应激( CUMS) 造模法干预，干预前后进行体质量测量、高架十字迷宫( EPM) 测试、旷场( OF) 试验、糖水试验、攻击行为测定，将结果与刚柔证核心证候比较，初步拟定切合证候表现的造模方法。结果: 干预完成后，打斗组及睡眠剥夺组攻击性行为及OF 试验总路程较对照组明显升高，其中打斗组总路程明显高于睡眠剥夺组。CUMS 组与束缚组大鼠糖水偏爱度明显下降，其中CUMS 组明显低于束缚组，CUMS 组大鼠体重、EPM 试验中开臂时间百分比、旷场试验总路程均明显下降。结论: 通过客观行为学检验评测，发现CUMS 及社交失败造模动物模型分别符合柔证及刚证核心症状，继而评定CUMS 及社交失败造模动物模型作为柔证及刚证大鼠造模方法。

Objective: evaluate four rigid-gentle syndrome model establishment methods and testify the scientific of this method by“Syndrome Identification from treatment”and repeat behavior experiment． Methods: separate 40 SD rats into five groups，which use sleeping deprivation method，social defeat method，restraint method and chronic unpredictable mile stress( CUMS) method to make intervention． Before and after the intervention，the rats will take some test include: EPM test，OF test，SUGAＲ test and attack behavior test． The prime method of rigid and gentle syndrome model is based on the relationship between the behavior result and clinic rigid－gentle syndrome core symptoms． Ｒesult: After the intervention，compared with control group，fighting group，sleep deprivation( SD) group’s aggressive behavior and moving distances in OF test are higher and the fighting group is higher than the SD group． Compared with control group，the Sugar water preference rate in CUMS group and restrict group is lower，and the CUMS group is lower than the restrict group while the percentage of time in open arms of the maze test，the weight and moving distances in CUMS is lower than the ones in the control group． Conclusion: This research found that the CUMS and improved solitary-social defeat modeling method are similar to the clinical core symptoms of gentle and rigid syndrome．

国家自然科学基金青年科学基金项目( 81704057) -基于LncＲNA-MALAT1 /miＲ-124 /BDNF 轴探讨疏肝方调控肝郁气滞证大鼠行为的表观遗传学机制; 国家科学技术部国际科技合作项目( 2009DFA31970) -中医心身医学刚柔辨证研究