Institutional Repository of Key Laboratory of Mental Health, CAS
Ionizing radiation alters functional neurotransmission in Drosophila larvae | |
Zhang, Yi1; Zhang, Yihao2,3; Shen, Cong4; Hao, Shun2,3; Duan, Wenlan2,3; Liu, Li2,3,5; Wei, Hongying2,3 | |
第一作者 | Zhang, Yi |
通讯作者邮箱 | weihongying@ibp.ac.cn (hongying wei) |
心理所单位排序 | 5 |
摘要 | IntroductionPatients undergoing cranial ionizing radiation therapy for brain malignancies are at increased risk of long-term neurocognitive decline, which is poorly understood and currently untreatable. Although the molecular pathogenesis has been intensively researched in many organisms, whether and how ionizing radiation alters functional neurotransmission remains unknown. This is the first study addressing physiological changes in neurotransmission after ionizing radiation exposure. MethodsTo elucidate the cellular mechanisms of radiation damage, using calcium imaging, we analyzed the effects of ionizing radiation on the neurotransmitter-evoked responses of prothoracicotropic hormone (PTTH)-releasing neurons in Drosophila larvae, which play essential roles in normal larval development. ResultsThe neurotransmitters dopamine and tyramine decreased intracellular calcium levels of PTTH neurons in a dose-dependent manner. In gamma irradiated third-instar larvae, a dose of 25 Gy increased the sensitivity of PTTH neurons to dopamine and tyramine, and delayed development, possibly in response to abnormal functional neurotransmission. This irradiation level did not affect the viability and arborization of PTTH neurons and successful survival to adulthood. Exposure to a 40-Gy dose of gamma irradiation decreased the neurotransmitter sensitivity, physiological viability and axo-dendritic length of PTTH neurons. These serious damages led to substantial developmental delays and a precipitous reduction in the percentage of larvae that survived to adulthood. Our results demonstrate that gamma irradiation alters neurotransmitter-evoked responses, indicating synapses are vulnerable targets of ionizing radiation. DiscussionThe current study provides new insights into ionizing radiation-induced disruption of physiological neurotransmitter signaling, which should be considered in preventive therapeutic interventions to reduce risks of neurological deficits after photon therapy. |
关键词 | ionizing radiation neurotransmission dopamine tyramine PTTH neurons Drosophila larvae |
2023-07-06 | |
语种 | 英语 |
DOI | 10.3389/fncel.2023.1151489 |
发表期刊 | FRONTIERS IN CELLULAR NEUROSCIENCE |
卷号 | 17页码:14 |
期刊论文类型 | 实证研究 |
收录类别 | SCI |
出版者 | FRONTIERS MEDIA SA |
WOS关键词 | LONG-TERM POTENTIATION ; SYNAPTIC PLASTICITY ; OXIDATIVE STRESS ; GENOME INTEGRITY ; ADULT SURVIVORS ; IRRADIATION ; CELLS ; MODEL ; LIPOPOLYSACCHARIDE ; BIOSYNTHESIS |
WOS研究方向 | Neurosciences & Neurology |
WOS类目 | Neurosciences |
WOS记录号 | WOS:001029427900001 |
WOS分区 | Q1 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.psych.ac.cn/handle/311026/45672 |
专题 | 中国科学院心理健康重点实验室 |
通讯作者 | Wei, Hongying |
作者单位 | 1.North China Res Inst Electroopt, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromolecules, State Key Lab Brain & Cognit Sci, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 4.China Elect Technol Grp Corp, Res Inst 45, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Psychol, CAS Key Lab Mental Hlth, Beijing, Peoples R China |
通讯作者单位 | 脑与认知科学国家重点实验室 |
推荐引用方式 GB/T 7714 | Zhang, Yi,Zhang, Yihao,Shen, Cong,et al. Ionizing radiation alters functional neurotransmission in Drosophila larvae[J]. FRONTIERS IN CELLULAR NEUROSCIENCE,2023,17:14. |
APA | Zhang, Yi.,Zhang, Yihao.,Shen, Cong.,Hao, Shun.,Duan, Wenlan.,...&Wei, Hongying.(2023).Ionizing radiation alters functional neurotransmission in Drosophila larvae.FRONTIERS IN CELLULAR NEUROSCIENCE,17,14. |
MLA | Zhang, Yi,et al."Ionizing radiation alters functional neurotransmission in Drosophila larvae".FRONTIERS IN CELLULAR NEUROSCIENCE 17(2023):14. |
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